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KMID : 0360120120340060384
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Journal of the Korean Society of Maxillofacial Plastic Reconstructive Surgeons
2012 Volume.34 No. 6 p.384 ~ p.390
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In vivo Osteogenesis of Cultured Human Periosteal-derived Cells and Polydioxanone/Pluronic F127 Scaffold
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Park Bong-Wook
Byun June-Ho
Lee Jin-Ho
Oh Se-heang
Kim Sang-June
Hah Young-Sool
Jeon Myoung-Hwa
Maeng Geun-Ho
Rho Gyu-Jin
Kim Jong-Ryoul
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Abstract
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Purpose: The purpose of this study is to examine in vivo osteogenesis of cultured human periosteal-derived cells and polydioxanone/pluronic F127 scaffold.
Methods: Two one-year-old miniature pigs were used in this study. $2{\times}10^6$ 수식 이미지 periosteal-derived cells in 1 mL medium were seeded by dropping the cell suspension into the polydioxanone/pluronic F127 scaffold. These cell-scaffold constructs were cultured in osteogenic Dulbecco’s modified Eagle’s medium for 7 days. Under general anesthesia with azaperone and tiletamine-zolazepam, the mandibular body and ramus of the pigs were exposed. Three bony defects were created. Polydioxanone/pluronic F127 scaffold with periosteal-derived cells and the scaffold only were implanted into each defect. Another defect was left empty. Twelve weeks after implantation, the animals were sacrificed.
Results: New bone formation was clearly observed in the polydioxanone/pluronic F127 scaffold with periosteal-derived cells. Newly generated bone was also observed in the scaffold without periosteal-derived osteoblasts and empty defect, but was mostly limited to the periphery.
Conclusion: These results suggest that cultured human periosteal-derived cells have good osteogenic capacity in a polydioxanone/pluronic F127 scaffold, which provides a proper environment for the osteoblastic differentiation of these cells.
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KEYWORD
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Periosteal-derived cells, Polydioxanone scaffold, In vivo osteogenesis
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